Cell wall disruption is necessary to maximize lipid extraction yields in conventional species of mass-cultivated microalgae. This study investigated the effect of sonication, solvent choice and number of extractions on the lipid yield, lipid class composition and fatty acid composition of the diatom <i>Porosira glacialis</i>. For comparison, the diatom <i>Odontella aurita</i> and green alga <i>Chlorella ...